armful to crops, including reduction of carbon assimilation in photosynthetic tissue (Munns and Tester, 2008; Ismail and Horie, 2017). As a result, mechanisms for Na+ exclusion from shoot are pivotal for the adaptation of plants in high-Na+ environments. Previous studies showed that Na+ retrieving from xylem sap inside the root is an essential physiological technique to achieve low shoots Na+ concentrations in the course of salt toxicity (Ismail and Horie, 2017; Zelm et al., 2020; Tian et al., 2021). This procedure is mediated by a variety of ion transporters (Horie et al., 2009; Zelm et al., 2020). The HKT1 family genes which encodes Na+ -selective transporters have already been demonstrated to play critical roles in this regulatory procedure. By way of example, Arabidopsis HKT1 is strongly expressed in root stelar cells and functions in shoots Na+ exclusion by retrieving Na+ from the xylem sap within the root (Sunarpi et al., 2005; Davenport et al., 2007; M ler et al., 2009). Moreover, the rice salt-tolerant QTL SKC1/Cathepsin L Synonyms OsHKT1;five, the wheat salt-tolerant QTLs Nax1/TmHKT1;4 and Nax2/TmHKT1;5, plus the maize salt-tolerant QTL ZmNC1/ZmHKT1 all encode HKT-type Na+ transporters that function similarly to Arabidopsis HKT1 (Ren et al., 2005; Huang et al., 2006; Byrt et al., 2007; Munns et al., 2012; Zhang et al., 2018). These research have showed that Na+ – DP supplier permeable HKT1 transporters mediate Na+ retrieving from xylem vessels and helpful for enhancement of salt tolerance. Aside from HKT1 household transporters, it remains largely unknown if other varieties transporters are also involved in retrieving Na+ from xylem vessels. Rice is often a staple food and its development and productivity are highly susceptible to salt tress (Ren et al., 2005; Ismail and Horie, 2017; Kobayashi et al., 2017). The genomes with the Nipponbare rice subspecies encode 27 OsHAK family members, four of which have been shown to mediate rice K+ /Na+ homeostasis for the duration of salt tension. By way of example, OsHAK1, OsHAK5, and OsHAK16 are induced by salt anxiety and involved in salt tolerance (Yang et al., 2014; Chen et al., 2015; Feng et al., 2019). OsHAK21 is crucial to keep Na+ /K+ homeostasis and market seed germination and seedling establishment beneath salinity tension (Shen et al., 2015; He et al., 2019). These research indicate that root K+ uptake mediated by HAK members of the family has good value for plant salt tolerance. Nonetheless, it remains unknown whether or not rice highaffinity K+ transport household (KT/HAK/KUP) members serves as Na+ transporters thereby functioning in salt tolerance in plants. When studying the function of OsHAK12 in rice, we located that OsHAK12, like many OsHAK members described above, was involved in salt tolerance as its mutants had been salt sensitive. Surprisingly, OsHAK12, as opposed to previously reported HAK members, failed to transport K+ but instead transported Na+ as assayed in yeast mutants. Constant with this transport activity, OsHAK12 apparently served as a Na+ – permeable transporter that retrieved Na+ from xylem back to root tissues and hence protected plants from salt toxicity by excluding Na+ from shoots.Supplies AND Strategies Plant Material and Growth ConditionsJaponica rice cultivar Nipponbare (O. sativa L.) was utilised as the wild kind within this study, as well as employed for the generation of all transgenic plant lines. IRRI (International Rice Study Institute) hydroponic resolution for rice was performed as earlier approach (Li et al., 2014; Wang et al., 2021). The modification of Na+ and K+ concentrations as indicated i