Name :
LAP (TGF-beta 1) Protein
Description :
Transforming growth factor beta (TGFβ) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGFβ gene product. Extracellular activation of these complexes is a critical step in regulation of TGFβ function in vivo. The TGFβ1 LAP is a ligand for the integrin αvβ6 and that αvβ6-expressing cells induce spatially restricted activition of TGFβ1. And LAP identifies a novel CD4+/CD25+ regulatory T cell subset with TGFbeta-mediated function and enhanced suppression of experimental autoimmune encephalomyelitis.
Species :
Human
Uniprotkb :
Human Cells
Tag :
N-6His-Avi
Synonyms :
transforming growth factor β1, TGFβ, CED, TGFB, TGFbeta, LAP (TGFβ 1), DPD1, LAP (TGFbeta 1), LAP (TGF-β 1), transforming growth factor beta 1, LAP, LAP (TGF-beta 1), TGFB1
Construction :
Biotinylated Recombinant Human Latency-associated Peptide (Transforming Growth Factor Beta-1) is produced by our Mammalian expression system and the target gene encoding Leu30-Arg278 (Cys33Ser) is expressed with a 6His, Avi tag at the N-terminus.
Protein Purity :
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight :
35-45 KDa, reducing conditions
Endotoxin :
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulatione :
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution :
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage :
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping :
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Research Background :
Transforming growth factor beta (TGFβ) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGFβ gene product. Extracellular activation of these complexes is a critical step in regulation of TGFβ function in vivo. The TGFβ1 LAP is a ligand for the integrin αvβ6 and that αvβ6-expressing cells induce spatially restricted activition of TGFβ1. And LAP identifies a novel CD4+/CD25+ regulatory T cell subset with TGFbeta-mediated function and enhanced suppression of experimental autoimmune encephalomyelitis.
References and Literature :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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