Name :
MD1 Protein
Description :
MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 15 (RP15) which is a molecule containing leucine-rich repeats and is expressed on B cells, dendritic cells, and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP15 cell surface expression and function. It is indicated that the RP15/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 4% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in the spleen, and also detectable in the liver, brain, thymus, and kidney.
Species :
Mouse
Uniprotkb :
HEK293
Tag :
His
Synonyms :
lymphocyte antigen 86, MD-1, MD1
Construction :
A DNA sequence encoding the extracellular domain (Met 1-Ser 162) of mouse LY86 (NP_034875.1) precursor was expressed with a C-terminal polyhistidine tag.
Protein Purity :
> 92 % as determined by SDS-PAGE
Molecular Weight :
Approxiamtely 18 kDa
Endotoxin :
Formulatione :
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 15 (RP15) which is a molecule containing leucine-rich repeats and is expressed on B cells, dendritic cells, and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP15 cell surface expression and function. It is indicated that the RP15/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 4% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in the spleen, and also detectable in the liver, brain, thymus, and kidney.
References and Literature :
1. Miura Y.,et al.,(1998), RP105 is associated with MD-1 and transmits an activation signal in human B cells. Blood 92:2815-2822. 2. Begum N.A.,et al., (1999), Human MD-1 homologue is a BCG-regulated gene product in monocytes: Its identification by differential display.Biochem. Biophys. Res. Commun. 256:325-329. 3. Mungall A.J.,et al.,(2003), The DNA sequence and analysis of human chromosome 6.Nature 425:805-811.
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