Name :
PRAK/MAPKAPK5 Protein
Description :
The p38 mitogen-activated protein kinase (MAPK) pathway plays an important role in cellular responses to inflammatory stimuli and environmental stress. p38 regulated/activated protein kinase (PRAK, also known as mitogen-activated protein kinase activated protein kinase 5 [MAPKAPK5]) functions downstream of p38alpha and p38beta in mediating the signaling of the p38 pathway.
Species :
Human
Uniprotkb :
Baculovirus-Insect Cells
Tag :
C-Strep-II
Synonyms :
MAPKAPK5, MK5, MAPKAP-K5, MK-5, MAPKAPK-5
Construction :
Recombinant Human PRAK Protein is expressed from Baculovirus-Insect Cells with Strep-II tag at the C-terminus. It contains Met1-Gln471.[Accession |NP_003659.2]
Protein Purity :
> 90% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight :
The protein has a predicted MW of 55.57 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin :
Less than 1EU per μg by the LAL method.
Formulatione :
Supplied as 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 2mM CaCl2(pH 8.0).
Reconstitution :
Stability & Storage :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
The p38 mitogen-activated protein kinase (MAPK) pathway plays an important role in cellular responses to inflammatory stimuli and environmental stress. p38 regulated/activated protein kinase (PRAK, also known as mitogen-activated protein kinase activated protein kinase 5 [MAPKAPK5]) functions downstream of p38alpha and p38beta in mediating the signaling of the p38 pathway.
References and Literature :
1. New L, et al. Regulation of PRAK subcellular location by p38 MAP kinases. Mol Biol Cell. 2003 Jun;14(6):2603-16. doi: 10.1091/mbc.e02-08-0538. Epub 2003 Mar 20. PMID: 12808055; PMCID: PMC194907.
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