Truncated recombinant T9 FctA (21-328) (pre-FctA), excluding the membrane spanning residues but retaining both the Cregion of the sign-peptide and the C-terminal sortase motif, was blended with N-terminally His-tagged T9 SipA36-173
At the amino acid sequence stage SipA is very conserved with virtually one hundred% identity inside all strains carrying the FCT types three and 4, but is a lot more…