The cells ended up transiently transfected employing the calcium phosphate approach or by using Lipofectin or Lipofectamine 2000 (Invitrogen) in accordance to the manufacturer’s protocols
Mobile viability was identified using the MTT assay. In brief, the cells had been plated at 26104 cells/properly in 96-effectively plates 24 h following transfection and incubated in medium (one…