Animal-Free Recombinant Human BDNF
BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical. Manufactured using all non-animal reagents.
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Source: |
E. Coli
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Purity:
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Above 98% as determined by SDS-PAGE Analysis.
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Cross Reactivity: |
Chicken, Frog, Guinea Pig, Hamster, Monkey, Mouse, Rat
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Biological Activity
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Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
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AA Sequence
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MHSDPARRG ELSVCDSIS EWVTAADKK TAVDMSGGT VTVLEKVPV SKGQLKQYF YETKCNPMG YTKEGCRGI DKRHWNSQC RTTQSYVRA LTMDSKKRI GWRFIRIDT SCVCTLTIK RGR
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| Research Interest: | , , Proliferation and Cell Cycle |
| Cell Type: | Neurons |
| Protein Cross Reactivity: | Chicken, Frog, Guinea Pig, Hamster, Monkey, Mouse, Rat |