Anti-Mouse CD86 (B7-2) PE-Cyanine7
The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein). CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity.
The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.
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Clone
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GL-1 (GL1)
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Format: |
PE-Cyanine7
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Applications
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FC
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Reactivity
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Mouse
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Isotype:
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Rat IgG2a, kappa
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| Cell Type: | , , , |
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Hathcock, K. S., Laszlo, G., Dickler, H. B., Bradshaw, J., Linsley, P., & Hodes, R. J. (1993). Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science, 262(5135), 905-907. Inaba, K., Witmer-Pack, M., Inaba, M., Hathcock, K. S., Sakuta, H., Azuma, M., … & Steinman, R. M. (1994). The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. The Journal of experimental medicine, 180(5), 1849-1860. Hathcock, K. S., Laszlo, G., Pucillo, C., Linsley, P., & Hodes, R. J. (1994). Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. The Journal of experimental medicine, 180(2), 631-640. |