N Ca dependent regulation from the inward K (K in) channels in pollen tubesdisruption of CPK or CPK entirely impaired Ca dependent inhibition of K in currents and enhanced pollen tube development (Zhao et al).UP vs.PI distinct DEGs were mainly involved in anxiety response and defense response (Supplemental File S) and had entirely diverse enriched GOterms from UP vs.Pc particular DEGs.As the mechanisms of SI and pathogen resistance (PR) are remarkably similar SI entails recognition and rejection of “self ” pollen grains and pathogen resistance (PR) requires recognition and rejection of pathogens (Hodgkin et al Sanabria et al Rea et al), therefore the GO term “immune system process” (GO) was additional analyzed and of UP vs.PI precise genes have been identified (Table).WRKY (a homolog of BnaAgD and BnaCgD) is a crucial transcriptional regulator of response to necrotrophic fungal pathogen HIF-2α-IN-1 custom synthesis Botrytis cinerea and Alternaria brassicicola infection in Arabidopsis, which requires crosstalk amongst jasmonateand salicylateregulated disease response pathways (Zheng et al Birkenbihl et al).The expression of AtCAFA (homolog of BnaCgD) is usually induced by a number of stressrelated hormones and stimuli.Mutation of AtCAFA triggered defective deadenylation of stressrelated mRNAs and lowered expression of pathogenesisrelated (PR) genes PR and PR, making plants extra susceptible to Pseudomonas syringae pv.tomato DC (Pst DC) infection (Liang et al).ERF (homolog of BnaAgD and BnaCgD) negatively regulates the expression of gene related to JAresponsive defense plus the resistance towards the necrotrophic fungal pathogen Fusarium oxysporum (McGrath et al).JAZ (homolog of BnaCgD), a member from the JAZ family, was a repressor of JA signaling pathways.WRKY (homolog of BnaAgDFrontiers in Plant Science www.frontiersin.orgMay PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21542610 Volume ArticleZhang et al.PollenStigma Interactions in Brassica napus L.TABLE Enriched UP vs.Computer particular DEGs in the GO term “pollination.” Brassica napus gene ID BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaAgD BnaCgD BnaCgD BnaCgD BnaCgD BnaCgD BnaCnngD Arabidopsis gene ID ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG Gene name Gene descriptionACA CPUORF ACT ANX None LIP None None CPK None ACA None ATROPGEF GLOX RALF PERK PERK ACA GLOX None NoneAutoinhibited Ca ATPase Conserved upstream opening reading frame relative to important ORF ATG.Belongs to the reproductive actin subclass that expressed in creating and reproductive tissues Receptorlike kinase required for maintenance of pollen tube development Unknown function Receptorlike cytoplasmic kinase that controls micropylar pollen tube guidance Protein kinase superfamily protein with octicosapeptidePhoxBemp domain Protein kinase superfamily protein with octicosapeptidePhoxBemp domain Member of Calcium Dependent Protein Kinase Unknown function Autoinhibited Ca ATPase Pollen Ole e allergen and extensin loved ones protein Encodes a member of KPPlike gene family, homolog of KPP (kinase partner protein) gene in tomato Glyoxal oxidaserelated protein Member of a diversely expressed predicted peptide loved ones displaying sequence similarity to tobacco Fast Alkalinization Issue (RALF) Encodes a member with the PERK household of putative receptor kinases Encodes a member from the PERK family of putative receptor kinases Autoinhibited Ca ATPase Glyoxal oxidaserelated protein Peroxidase superfamily protein Pollen Ole e allergen and extensin household proteinand Bn.