Name : TNF-α, Mouse (P. pastoris-expressed)

Product Concentration :

Background :
Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.

Biological Activity :
ED50 1.0 × 108 units/mg.

expression system :
P. pastoris

Tag :
TNF-α (Ser84-Leu235)Accession # P06804

Protein Accession No. :
P06804

Synonyms :
TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

Molecular Weight :
~17 kDa, on SDS-PAGE under reducing conditions.

Purity :
> 95% as analyzed by SDS-PAGE

Product Concentration :
Lyophilized after extensive dialysis against PBS.

Storage and Stability :
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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