Name : MIP-1α/CCL3, Mouse

Product Concentration :

Background :
MIP-1 Alpha, also known as CCL3, G0S19-1 and SCYA3, LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. It binds to CCR1, CCR4 and CCR5, and participates in the host response to invading pathogens by regulating the trafficking and activation of inflammatory cells, such as macrophages, lymphocytes, NK cells and dendritic cells. MIP-1 alpha polymorphisms are associated with HIV susceptibility or resistance. Recombinant MIP-1 alpha induces a dose-dependent inhibition of HIV and SIV infection. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. Additionally, in wounds, MIP-1α chemoattracts macrophages in order to accelerate the tissue repair process.

Biological Activity :
The EC50 value of mouse MIP-1α/CCL3 on Ca2+ mobilization assay in CHO-K1/Gα15/mCCR1 cells (human Gα15 and mouse CCR1 stably expressed in CHO-K1 cells) is less than 100.0 ng/ml.

expression system :
HEK 293

Tag :
MIP-1α (Ala24-Ala92)Accession # Q5QNW0

Protein Accession No. :

Synonyms :
Macrophage Inflammatory Protein-1α; CCL-3; LD78α; LD78 alpha

Molecular Weight :
~7.8 kDa, on SDS-PAGE under reducing conditions.

Purity :
> 95% as analyzed by SDS-PAGE> 95% as analyzed by HPLC

Product Concentration :
Lyophilized after extensive dialysis against PBS.

Storage and Stability :
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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