Animal-Free Recombinant Human IL-10
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Manufactured using all non-animal reagents.
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Source: |
E. Coli
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Purity:
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Above 98% as determined by SDS-PAGE Analysis.
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Cross Reactivity: |
Bacteria, Monkey, Mouse, Virus
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Biological Activity
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Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
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AA Sequence
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MSPGQGTQS ENSCTHFPG NLPNMLRDL RDAFSRVKT FFQMKDQLD NLLLKESLL EDFKGYLGC QALSEMIQF YLEEVMPQA ENQDPDIKA HVNSLGENL KTLRLRLRR CHRFLPCEN KSKAVEQVK NAFNKLQEK GIYKAMSEF DIFINYIEA YMTMKIRN
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| Research Interest: | , , , , , Inflammation |
| Cell Type: | , , , |
| Protein Cross Reactivity: | Bacteria, Monkey, Mouse, Virus |