Cultured SH-SY5Y and CCF-STTG1 cells were incubated at 42uC for one hour ahead of harvesting and sequential extraction to independent soluble and insoluble proteins (see Strategies and Determine 1)
To boost statistical energy for G-test evaluation of proteins discovered with high-confidence (99% protein self-assurance, ninety five% peptide self-confidence and made up of 2 unique peptides) we provided in the…