Quantitative PCR reactions had been carried out making use of a qPCR SYBR Green Package (Finzymes) and a Rotor-Gene RG-3000 actual-time thermocycler (Corbett Analysis), with a final reaction quantity of twenty mL

The exercise of caspase-3, a main executioner protease involved in the apoptotic process, was established by launch of 7-amino-4trifluoromethyl-coumarin (AFC) from a DEVD-AFC peptide substrate (Sigma). Cells (26106), both handle…