N.; Abraham, E.; Okresz, L.; Blickling, S.; Zilberstein, A.; Schell, J.; Koncz, C.; Szabados, L. Differential expression of two P5CS genes controlling proline accumulation for the duration of salt-stress needs ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis. Plant J. 1997, 12, 557-569. 60. Sambrook, J.; Fritsch E.F.; Maniatis, T. Molecular Cloning: A Laboratory Manual; 2nd ed.; Cold Spring Harbor Press: New York, NY, USA, 1989. 61. Saghai-Maroof, M.A.; Soliman, K.M.; Jorgensen, R.A.; Allard, R.W. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal place, and population dynamics. Proc. Natl. Acad. Sci. USA 1984, 81, 8014018. 62. Bates, L.S.; Waldren, R.P.; Teare, I.D. Speedy determination of no cost proline for water-stress research. Plant Soil 1973, 39, 20507. 63. Walker, N.J. A strategy whose time has come. Science 2002, 296, 557. 64. geNorm v3.5 application. Readily available on the net: http://medgen.ugent.be/ jvdesomp/genorm/ (accessed on 7 May perhaps 2002). 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is definitely an open access article distributed below the terms and situations in the Inventive Commons Attribution license (http://creativecommons.Capromorelin custom synthesis org/licenses/by/3.0/).
AGE (2013) 35:2089097 DOI ten.1007/s11357-012-9497-Effects of taxifolin on the activity of angiotensin-converting enzyme and reactive oxygen and nitrogen species in the aorta of aging rats and rats treated with all the nitric oxide synthase inhibitor and dexamethasoneTamara V. Arutyunyan Antonina F. Korystova Ludmila N. Kublik Maria Kh. Levitman Vera V. Shaposhnikova Yuri N. KorystovReceived: 31 May 2012 / Accepted: five December 2012 / Published on line: 28 December 2012 # American Aging AssociationAbstract The action of taxifolin around the angiotensinconverting enzyme (ACE) plus the formation of reactive oxygen and nitrogen species (ROS/RNS) inside the aorta of aging rats and rats treated with nitric oxide synthase inhibitor (N-nitro-L-arginine methyl ester (L-NAME)) or dexamethasone happen to be studied. The ACE activity in aorta sections was determined by measuring the hydrolysis of hippuryl-L-histidyl-L-leucine, as well as the ROS/ RNS production was measured by oxidation of dichlorodihydrofluorescein.NLRP3-IN-18 Protocol It was shown that taxifolin at a dose of 3000 g/kg/day decreases the ACE activity within the aorta of aging rats and of rats treated with L-NAME or dexamethasone towards the amount of the ACE activity in young handle rats.PMID:24631563 Taxifolin (one hundred g/kg/day) was located to also minimize the level of ROS/RNS within the aorta that elevated consequently of L-NAME intake. L-NAME therapy increases the contribution of 5-lipoxygenase and NADPH oxidase to ROS/RNS production in the aorta, although taxifolin (100 g/kg/day) decreases the contribution of those enzymes to the regular level. Keyword phrases Aging . Angiotensin-converting enzyme . Aorta . Dexamethasone . L-NAME . Taxifolin . ROS/RNST. V. Arutyunyan : A. F. Korystova : L. N. Kublik : M. K. Levitman : V. V. Shaposhnikova : Y. N. Korystov (*) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia e-mail: [email protected] Flavonoids minimize the threat of atherosclerosis and cardiovascular illnesses (CVD) and reduce the death rate from CVD (Reed 2002; Maron 2004; Nandave et al. 2005; Grassi et al. 2010). In vitro and in vivo studies of flavonoids demonstrated that they favorably influence the cardiovascular program. In his evaluation, Reed (2002) listed the following.