Anti-Human CD86 (B7-2) SAFIRE Purified
The IT2.2 antibody reacts with human CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
The IT2.2 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells. It is reported to be cross-reactive with Rhesus, Cynomolgus and Common marmoset CD86.
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Clone
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IT2.2
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Format: |
SAFIRE Purified
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Applications
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FC, FA
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Reactivity
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Human
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Isotype:
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Mouse IgG2b, kappa
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Preperation:
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The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography.
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Formulation:
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Phosphate-buffered aqueous solution, ph7.2.
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References:
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Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989. Azuma, M., Ito, D., Yagita, H., Okumura, K., Phillips, J. H., Lanier, L. L., & Somoza, C. (1993). B70 antigen is a second ligand for CTLA-4 and CD28.Nature, 366(6450), 76-79. Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998. |