Recombinant Human IGF-BP5
IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP5 is a 28.6 kDa cysteine-rich secreted protein produced by vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP-5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP-5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Recombinant human IGF-BP5 is a 28.6 kDa protein consisting of 253 amino acid residues. MPTP (hydrochloride)
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Source: |
E. Coli
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Purity:
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Above 98% as determined by SDS-PAGE Analysis.
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Cross Reactivity: |
NULL
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Biological Activity
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The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is ≤ 0.3 μg/ml in the presence of 15 ng/ml of human IGF-II.
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AA Sequence
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MLGSFVHCE PCDEKALSM CPPSPLGCE LVKEPGCGC CMTCALAEG QSCGVYTER CAQGLRCLP RQDEEKPLH ALLHGRGVC LNEKSYREQ VKIERDSRE HEEPTTSEM AEETYSPKI FRPKHTRIS ELKAEAVKK DRRKKLTQS KFVGGAENT AHPRIISAP EMRQESEQG PCRRHMEAS LQELKASPR MVPRAVYLP NCDRKGFYK RKQCKPSRG RKRGICWCV DKYGMKLPG MEYVDGDFQ CHTFDSSNV S
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| Research Interest: | , , Proliferation and Cell Cycle, |
| Protein Cross Reactivity: | NULL |