nuscript.Funding: The authors are extremely grateful for the Office of Research Assistance at Qatar University (grant (grant Funding: The authors are very grateful to the Workplace of Analysis Assistance at Qatar University no. QUCGCPH20/211) and also the Qatar Foundation–Qatar National Research Fund (UREP27160 no. QUCG-CPH-20/21-1) and the Qatar Foundation–Qatar National Analysis Fund (UREP27-160-33045), to H.M.K., for funding this operate. 045), to H.M.K., for funding this work. Acknowledgments: Figures 1 and three have been generated employing publicly available tools from Servier Servier Acknowledgments: Figures 1 and 3 had been generated making use of publicly obtainable tools from Medical Art (Wise), which can be licensed beneath a Inventive Commons Attribution three.0 Unported License Health-related Art (Clever), that are licensed below a Creative Commons Attribution three.0 Unported License and vectors from Freepik (freepik, accessed on 16 June 2021).and vectors from Freepik (freepik, accessed on 16 June 2021). Conflicts of Interest: The authors declare no conflict of interest.Conflicts of Interest: The authors declare no conflict of interest.
International Journal ofMolecular SciencesArticleComparing Early Transcriptomic Responses of 18 Soybean (Glycine max) Genotypes to Iron StressDaniel R. Kohlhase 1 , ATR Activator Source Chantal E. McCabe two, , Asheesh K. Singh 1 , Jamie A. O’Rourke 2, and Michelle A. Graham two, Department of Agronomy, Iowa State University, Ames, IA 50011, USA; [email protected] (D.R.K.); [email protected] (A.K.S.) U.S. Division of Agriculture (USDA)–Agricultural Research Service (ARS), Corn Insects and Crop Genetics Analysis Unit, Ames, IA 50011, USA; [email protected] Correspondence: [email protected] (J.A.O.); [email protected] (M.A.G.) Present address: Division of Quantitative Health Sciences, Mayo Clinic, Rochester, MN 55901, USA.Citation: Kohlhase, D.R.; McCabe, C.E.; Singh, A.K.; O’Rourke, J.A.; Graham, M.A. Comparing Early Transcriptomic Responses of 18 Soybean (Glycine max) Genotypes to Iron Tension. Int. J. Mol. Sci. 2021, 22, 11643. doi.org/10.3390/ ijms222111643 Academic Editor: Anna M. Mastrangelo Received: 23 September 2021 Accepted: 25 October 2021 Published: 28 OctoberAbstract: Iron deficiency chlorosis (IDC) is an abiotic pressure that negatively impacts soybean (Glycine max [L.] Merr.) production. Considerably of our expertise of IDC pressure responses is derived from model plant species. Gene expression, quantitative trait loci (QTL) mapping, and genome-wide association studies (GWAS) performed in soybean suggest that anxiety response differences exist among model and crop species. Our current understanding on the molecular response to IDC in soybeans is largely derived from gene expression research working with near-isogenic lines differing in iron efficiency. To enhance iron efficiency in soybeans and other crops, we ought to expand gene expression studies to involve the diversity present in germplasm collections. Thus, we collected 216 purified RNA samples (18 genotypes, two tissue varieties [leaves and roots], two iron therapies [sufficient and deficient], 3 replicates) and utilised RNA sequencing to examine the expression variations of 18 diverse soybean genotypes in response to iron deficiency. We found a speedy response to iron deficiency GlyT2 Inhibitor custom synthesis across genotypes, most responding inside 60 min of anxiety. There was little proof of an overlap of specific differentially expressed genes, and comparisons of gene ontology terms and transcription aspect famil