Name : SPARC, Human

Product Concentration :

Background :
SPARC, an acronym for ’secreted protein, acidic and rich in cysteine’, is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid , 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.

Biological Activity :
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 μg/ml, corresponding to a specific activity of > 333.0 IU/mg.

expression system :
E. coli

Tag :
Expressed with additional N-terminal sequence (MSYYHHHHHH DYDIPTTENLYFQGAMGS). SPARC (Ala18-Ile303)Accession # P09486

Protein Accession No. :

Synonyms :
Basement-membrane protein 40; BM-40; Osteonectin; Secreted protein acidic and rich in cysteine

Molecular Weight :

Purity :
> 95% as analyzed by SDS-PAGE> 95% as analyzed by HPLC

Product Concentration :
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Storage and Stability :
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

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