Name : VEGF164, Mouse
Product Concentration :
Background :
Vascular Endothelial Growth Factor (VEGF) was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer of KDR/Flt in VEGF signal transduction remain to be elucidated. In vivo, VEGF has been found to be a potent angiogenesis inducer.
Biological Activity :
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
expression system :
E. coli
Tag :
Expressed with an N-terminal Met. VEGF164 (Ala27-Arg190)Accession # Q00731-2
Protein Accession No. :
Q00731-2
Synonyms :
Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen; VEGF-164; Vascular Permeability Factor; VPF
Molecular Weight :
Purity :
> 95% as analyzed by SDS-PAGE> 95% as analyzed by HPLC
Product Concentration :
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Storage and Stability :
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.
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