Product Name :
Human IL-15RA&IL-15 Protein
EN
IL-15 R alpha；CD215；IL15RA；IL-15RA；IL-15R-alpha1；Interleukin-15；IL-15；IL15
Description:
| Human IL-15RA&IL-15 Protein with the C-His Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human IL-15RA&IL-15 Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Source:
The recombinant Human IL-15RA&IL-15 Protein, (amino acids Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight :
The recombinant Human IL-15RA&IL-15 protein with the His tag at the C-terminus has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result.
Activity:
Immobilized recombinant Human IL-15RA&IL-15 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-2 R beta&IL-2 R gamma protein with the C-hFc tag with the EC50 of 38.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.
Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human IL-15RA&IL-15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping:
The recombinant Human IL-15RA&IL-15 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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