Product Name :
Human IL-23 alpha&Mouse IL-12 beta Protein

EN
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta

Description:
| Human IL-23 alpha&Mouse IL-12 beta Protein with the C-His-Avi Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.

Source:
The recombinant Human IL-23 alpha&Mouse IL-12 beta Protein, (amino acids Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.

Molecular Weight :
The recombinant Human IL-23 alpha&Mouse IL-12 beta protein with the His-Avi tag at the C-terminus has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Tris-Bis PAGE result.

Activity:
Immobilized recombinant Human IL-23 alpha&Mouse IL-12 beta protein with the C-His-Avi tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-23R protein with the C-mFc tag with the EC50 of 0.15 ug/ml determined by ELISA.

Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.

Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.

Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human IL-23 alpha&Mouse IL-12 beta Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping:
The recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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