Pended in 50 ml of ultra-pure water, and the DNA was stored
Pended in 50 ml of ultra-pure water, and the DNA was stored at 220uC. PCR was performed according to Britto et al. (1995), with 7.5 ml of the extracted DNA…
Ch is placed underneath a grid and, therefore, cannot be removed
Ch is placed Ntrol Control Control Control ControlmiRNA pattern A A A A A underneath a grid and, therefore, cannot be removed by the animals. In addition, each cylinder is…
Type or genotype (TREC) analysis in the purified CD34+ population used
Type or genotype (TREC) analysis in the purified CD34+ population used for seeding the matrix. Secondly our sjTREC analysis of cells generated in matrix cultures revealed values of about 1.5…
More emphasis on pathways whose member genes show fold changes that
More emphasis on pathways whose member genes show fold changes that are congruent with underlying interaction topology. We also performed the GO analysis to supplement limited information obtained from IF's…
N contrast, we observed that UC-MSCs educated CD4+CD25+ T regulatory
N contrast, we observed that UC-MSCs educated CD4+CD25+ T regulatory cells exerted a significant adverse tendency in the plasma level of interferon- compared to those receiving PBS (Figure 2C, p
Rame Slides (MDS Inc., Ontario, Canada) then stained and fixed according
Rame Slides (MDS Inc., Ontario, Canada) then stained and fixed according to the HistoGeneTM LCM Frozen Section Staining Kit protocol (MDS Inc.). For each sample approximately 2000 cells were laser…
Followed by infection with Lm-gp61 one day later. Following Lm-gp61 infection
Followed by infection with Lm-gp61 one day later. Following Lm-gp61 infection, WT SMARTA cells expanded, contracted and disappeared from the memory pool within a few weeks, as previously 10781694 reported…
Bs forms the costal bones of the carapace and likewise appears
Bs forms the costal bones of the carapace and likewise appears to be mediated by well described genetic networks acting outside of their canonical vertebrate developmental compartments. The bone morphogenetic…
N miRNA target sequence into the 39-UTR of reporter genes and
N miRNA target sequence into the 39-UTR of reporter genes and containing two independent expression cassettes encoding Gaussia luciferase (Gluc) and firefly luciferase (Fluc). Using the Asensors, miRNA activity can…
Non-specific binding. The blot was subsequently incubated with an anti-HIF-1a
Non-specific binding. The blot was subsequently incubated with an anti-HIF-1a rabbit polyclonal antibody (1:200, Abcam), an anti-Sost rabbit polyclonal antibody (1:60, Abcam), or an anti-HSP90 rabbit polyclonal antibody (1:200, Abcam)…