Mation Table 4). Electrophoretic mobility shift assays (EMSA) demonstrated binding of GST-OsGRF4 to DNA fragments containing intact but not mutant GCGG core motifs (Fig. 3h), and ChIP-PCR confirmed in vivo association of OsGRF4 with GCGG-containing promoter fragments from multiple NH4+ Mavorixafor In Vivo metabolism genes, which includes OsAMT1.1 and OsGS1.two (Fig. 3i; Extended Information Fig. 2k-n). Finally, OsGRF4 activates transcription from OsAMT1.1 and OsGS1.two promoters in transactivation assays (Fig. 3j, k; Extended Data Fig. 2o). Additional experiments demonstrated that OsGRF4mediated transcriptional activation also promotes NO3- metabolism (Fig. 3b, c; Extended Data Fig. 3). As a result, OsGRF4 is definitely an all round transcriptional activator of N metabolism, and counteracts the inhibitory effects of SLR1. We next investigated how GA, SLR1, and OsGRF4 regulate N metabolism. GA promotes both NJ6 and NJ6-sd1 15NH4+ uptake rates to similarly high levels (Fig. 4a). Also, the GAbiosynthesis inhibitor pacolubutrazol25 (PAC) reduces NJ6 and NJ6-sd1 15NH4+ uptakes, whilst GA restores it (Fig. 4a). Thus, SLR1 accumulation (as a consequence of sd1 or PAC) reduces NH4+ uptake, whilst SLR1 reduction (on account of GA) increases it. Furthermore, the GA-DELLA technique differentially regulates the abundance of NH4+ metabolism mRNAs: OsAMT1.1 and OsGS1.2 mRNA abundances are elevated by GA, reduced by PAC, and restored by combined GA and PAC (Fig. 4b). We next identified that PAC reduces, whilst GA promotes ChIP-PCR enrichment of GCGG motif-containing fragments in the OsAMT1.1 and OsGS1.2 promoters (Fig. 4c). Therefore, SLR1 accumulation inhibits, whilst SLR1 reduction promotes binding of OsGRF4 to OsAMT1.1 and OsGS1.two promoters (Fig. 4c), thereby affecting mRNA abundance and NH4+ metabolism (Fig. 4a, b; Extended Data Fig. 4a, b). SLR1 abundance also likely affects NO3- uptake (Fig. 3b; Extended Information Fig. 3a, b) and NR activity (Fig. 3c; Extended Data Fig. 4c) by way of inhibition of OsGRF4 activation of NO3metabolism genes. Whilst interaction of OsGRF4 with OsGIF (GRF-interacting issue) co-activators by way of a conserved QLQ domain (Extended Information Fig. 5a, b) promotes target gene expression18,Nature. Author manuscript; available in PMC 2019 February 15.Li et al.Pagebimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP) assays 3cl protease Inhibitors MedChemExpress revealed that SLR1 interferes with this interaction (Fig. 4d, e; Extended Data Fig. 5c). In vivo fluorescence resonance power transfer (FRET) assays demonstrated that SLR1 competitively inhibits the OsGRF4-OsGIF1 interaction, and that GA relieves this inhibition (Fig. 4f, g). Whilst the OsGRF4-OsGIF1 interaction promotes binding of OsGRF4 to GCGG motif-containing DNA fragments, SLR1 inhibits this promotion by inhibiting the OsGRF4OsGIF1 interaction (but will not directly interfere with all the DNA-binding of OsGRF4; Fig. 4h). Accordingly, SLR1 inhibits OsGRF4-OsGIF1-mediated transactivation from OsAMT1.1 and OsGS1.2 promoters (Fig. 4i). Importantly, OsGRF4 abundance is self-promoted, and SLR1 inhibits that promotion. Whilst OsGRF4 mRNA abundance is reduced in NJ6-sd1 (versus NJ6) but improved in NJ6-sd1OsGRF4ngr2 (versus NJ6-sd1; Extended Data Fig. 6a), GA increases OsGRF4 mRNA abundance, and overcomes PAC-mediated reductions in OsGRF4 mRNA abundance (Extended Information Fig. 6b). Moreover, OsGRF4 binds in vivo with GCGG-containing OsGRF4 promoter fragments (Extended Information Fig. 6c), and SLR1 inhibits OsGRF4-OsGIF1mediated transcriptional activation of your OsGRF4 promoter (Extended Information Fig.