Epresentative of OsGRF4 promoter haplotypes A, B and C (see key text) are shown. e, OsGRF4 mRNA Glycodeoxycholic Acid Metabolic Enzyme/Protease abundance in many rice varieties under the high N conditions (1.25 mM NH4NO3), OsGRF4 promoter haplotypes as indicated. Abundance information is all relative for the abundance of rice Actin2 mRNA. Information shown as imply s.e.m. (n = three). Diverse letters denote considerable variations (P 0.05, Duncan’s multiple variety test). f, Comparisons of OsGRF4 mRNA abundance in chosen rice varieties grown in involving higher (HN, 1.25 mM NH4NO3) and low (LN, 0.375 mM NH4NO3) N situations. Information shown as mean s.e.m. (n = three). Abundance data is all relative to that in HN (set to one). P 0.05 as when compared with HN by two-sided Student’s ttest. g, Relative abundances of rice OsmiR396 members of the family in NJ6 plants grown at different levels of N provide (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3), shown relative to abundance in plants grown in 1N situations (set to one particular). Data shown as imply s.e.m. (n = three). Diverse letters denote considerable differences (P 0.05, Duncan’s numerous range test).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsNature. Author manuscript; available in PMC 2019 February 15.Li et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure 2. Comparisons NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 isogenic line traits reveals that OsGRF4 regulates expression of NH4+ metabolism genes.a, Mature plant height. Data shown as imply s.e.m. (n = 16). Different letters denote important variations (P 0.05, Duncan’s a number of range test). b, The amount of tillers per plant. c, The number of grains per panicle. Data shown as mean s.e.m. (n = 16). Distinct letters denote substantial variations (P 0.05, Duncan’s various variety test). d, Flag-leaf width. Data shown as mean s.e.m. (n = 16). Diverse letters denote important variations (P 0.05, Duncan’s numerous range test). e, Culm (stem) width expressed as diameter of the uppermost internode. Data shown as imply s.e.m. (n = 16). Unique letters denote considerable differences (P 0.05, Duncan’s multiple range test). f, Grain yield per plant. Data shown as mean s.e.m. (n = 220). Distinctive letters denote considerable variations (P 0.05, Duncan’s several variety test). g, Relative root abundance of OsAMT1.two mRNA in NILs, genotypes as indicated. Abundance shown relative to that in NJ6 plants (=1). Information shown as mean s.e.m. (n = 3). Distinctive letters denote substantial variations (P 0.05, Duncan’sNature. Author manuscript; offered in PMC 2019 February 15.Li et al.Pagemultiple range test). h, Root glutamine synthase (GS) activities. Data shown as imply s.e.m. (n = 3). Various letters denote important differences (P 0.05, Duncan’s several range test). i, Relative shoot abundance of OsFd-GOGAT mRNA. Abundance shown relative to that in NJ6 plants (=1). Data shown as imply s.e.m. (n = three). Different letters denote considerable variations (P 0.05, Duncan’s multiple variety test). j, Shoot glutamine synthase (GS) activities. Data shown as imply s.e.m. (n = three). Distinct letters denote significant differences (P 0.05, Duncan’s various range test). k-n, Flag-OsGRF4 mediated ChIP-PCR D-?Carvone Data Sheet enrichment (relative to input) of GCGG-containing promoter fragments (marked with ) from OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters. Diagrams depict putative OsAMT1.two, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters.